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Western blot analysis of extracts of various cell lines, using ZAK antibody (A14513) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
ZAK Rabbit pAb
Catalog No：AB-A14513
Manufacturer：ABclonal
Synonyms：MAP3K20;AZK;MLK7;MLT;MLTK;MLTKalpha;MLTKbeta;MRK;SFMMP;ZAK;mlklak;pk
Applications：WB,IF
Reactivity：Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ZAK Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-312 of human ZAK (NP_598407.1). |
| Gene ID: | 51776 |
| Swiss prot: | |
| Molecular weight: | 35kDa/51kDa/91kDa |
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