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Western blot analysis of extracts of various cell lines, using PRAME antibody (A14507) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
PRAME Rabbit pAb
Catalog No：AB-A14507
Manufacturer：ABclonal
Synonyms：PRAME;CT130;MAPE;OIP-4;OIP4
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PRAME Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRAME (NP_006106.1). |
| Gene ID: | 23532 |
| Swiss prot: | P78395 |
| Molecular weight: | 57kDa |
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