Western blot analysis of extracts of various cell lines, using PDIA5 antibody (A14389) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 30s.
PDIA5 Rabbit pAb
Catalog No:AB-A14389
Manufacturer:ABclonal
Synonyms:PDIA5;PDIR
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PDIA5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. The N-terminal TRX-like domain is the primary binding site for the major ER chaperone calreticulin and possibly other proteins and substrates as well. Alternative splicing results in multiple protein- and non-protein-coding transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-140 of human PDIA5 (NP_006801.1). |
Gene ID: | 10954 |
Swiss prot: | Q14554 |
Molecular weight: | 30kDa/59kDa |
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