Western blot analysis of extracts of various cell lines, using DEF6 antibody (A14337) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
DEF6 Rabbit pAb
Catalog No:AB-A14337
Manufacturer:ABclonal
Synonyms:DEF6;IBP;SLAT;SWAP70L
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DEF6 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).[supplied by OMIM, Mar 2008] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-330 of human DEF6 (NP_071330.3). |
Gene ID: | 50619 |
Swiss prot: | Q9H4E7 |
Molecular weight: | 73kDa |
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