Western blot analysis of extracts of various cell lines, using GPNMB antibody (A14270) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
GPNMB Rabbit pAb
Catalog No:AB-A14270
Manufacturer:ABclonal
Synonyms:GPNMB;HGFIN;NMB
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GPNMB Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-260 of human GPNMB (NP_001005340.1). |
Gene ID: | 10457 |
Swiss prot: | Q14956 |
Molecular weight: | 62kDa/63kDa |
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Reactivity:Human,Mouse,Rat
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Conjugate:No
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Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
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Applications:WB,IF
Reactivity:Human
Host:Rabbit
Conjugate:No
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