Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (A14225) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Cyclin E1 Rabbit pAb
Catalog No:AB-A14225
Manufacturer:ABclonal
Synonyms:CCNE1;CCNE;pCCNE1;cyclin E1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Cyclin E1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-410 of human Cyclin E1 (NP_001229.1). |
Gene ID: | 898 |
Swiss prot: | P24864 |
Molecular weight: | 47kDa |
Help & Support
Product
Guarantee and
Expert Support
Related products
Catalog No:AB-A0146
Manufacturer:ABclonal
Synonyms:SLC9A3R1;EBP50;NHERF;NHERF-1;NHERF1;NPHLOP2
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Catalog No:AB-A0150
Manufacturer:ABclonal
Synonyms:TRAF1;EBI6;MGC:10353
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Catalog No:AB-A0139
Manufacturer:ABclonal
Synonyms:SPHK1;SPHK
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Catalog No:AB-A0038
Manufacturer:ABclonal
Synonyms:BRAF;B-RAF1;B-raf;BRAF1;NS7;RAFB1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Catalog No:AB-A0047
Manufacturer:ABclonal
Synonyms:DHQU;DIA4;DTD;NMOR1;NMORI;QR1;NQO1
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Catalog No:AB-A0028
Manufacturer:ABclonal
Synonyms:DYT14;DYT5b;TYH;TH
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00