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Western blot analysis of extracts of various cell lines, using Vinculin antibody (A14193) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Vinculin Rabbit pAb
Catalog No：AB-A14193
Manufacturer：ABclonal
Synonyms：VCL;CMD1W;CMH15;HEL114;MV;MVCL;vinculin
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Vinculin Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 767-1066 of human Vinculin (NP_003364.1). |
| Gene ID: | 7414 |
| Swiss prot: | P18206 |
| Molecular weight: | 24kDa/116kDa/123kDa |
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