Western blot analysis of extracts of various cell lines, using NANOG antibody (A14150) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 120s.
NANOG Rabbit pAb
Catalog No:AB-A14150
Manufacturer:ABclonal
Synonyms:NANOG
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NANOG Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NANOG (NP_079141.2). |
Gene ID: | 79923 |
Swiss prot: | Q9H9S0 |
Molecular weight: | 32kDa/34kDa |
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