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Recombinant Human IL-12 Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 37 kDa and 40-55 kDa.
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Immobilized Recombinant Human IL-12 at 1μg/mL (100 μL/well) can bind Recombinant Human IL12Rβ1 with a linear range of 30.6-122.4 ng/mL.
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Recombinant Human IL-12 promotes the proliferation of anti-CD3 antibody activated Human T lymphocyte leukemia cells (jurkat). The ED50 for this effect is typically 0.1-0.4 ng/mL.
Recombinant Human IL-12 Protein
Catalog No：AB-RP01232
Manufacturer：ABclonal
Synonyms：CLMF,IL12,p70,Interleukin-12,IL12A,P35,NKSF
Expression Host：HEK293 cells
€60,00 – €620,00
| Product Name: | Recombinant Human IL-12 Protein |
| Category: | Proteins/Peptides > Interleukin |
| Description: | Recombinant Human IL-12 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile23-Ser328&Arg23-Ser219) of human IL-12 (Accession #NP_002178.2/NP_000873.2) fused with a 6xHis tag at the C-terminus(IL12B),Flag tag at the C-terminus(IL12A). |
| Gene ID: | 6733593/3592 |
| Swiss prot: | P29460/P29459 |
| Bioactivity: | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IL-12 at 1μg/mL (100 μL/well) can bind Recombinant Human IL12Rβ1 with a linear range of 30.6-122.4 ng/mL.|Measured in a cell proliferation assay using anti-CD3 antibody activated Human T lymphocyte leukemia cells (jurkat). The ED50 for this effect is typically 0.1-0.4 ng/mL. |
| Endotoxin: | Please contact us for more information. |
| Reconstitution: | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
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