Western blot analysis of extracts of various cell lines, using Histone H3.3 antibody (A13824) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Histone H3.3 Rabbit pAb
Catalog No:AB-A13824
Manufacturer:ABclonal
Synonyms:H3F3A;H3.3A;H3F3;histone H3.3
Applications:WB
Reactivity:Human,Mouse,Rat,Other (Wide Range)
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Histone H3.3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human H3F3A (NP_002098.1). |
Gene ID: | 3020 |
Swiss prot: | P84243 |
Molecular weight: | 15kDa |
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