Western blot analysis of extracts of various cell lines, using NDUFB4 antibody (A13820) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts from normal (control) and NDUFB4 knockout (KO) HeLa cells, using NDUFB4 antibody (A13820) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] NDUFB4 Rabbit pAb
Catalog No:AB-A13820
Manufacturer:ABclonal
Synonyms:NDUFB4;B15;CI-B15
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] NDUFB4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1). |
Gene ID: | 4710 |
Swiss prot: | O95168 |
Molecular weight: | 14kDa/15kDa |
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