Recombinant Human EGF Protein
Catalog No:AB-RP01030
Manufacturer:ABclonal
Synonyms:Epidermal Growth Factor;HOMG4;URG
Expression Host:HEK293 cells
€34,00 – €215,00
Product Name: | Recombinant Human EGF Protein |
Category: | Proteins/Peptides > Growth factor,Cell Culture related |
Description: | Recombinant Human EGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn 971-Arg 1023) of human EGF (Accession #NP_001954.2) fused with an Fc, 6xHis tag at the C-terminus. |
Gene ID: | 4901950 |
Swiss prot: | P01133 |
Bioactivity: | Measured by its ability to stimulate EGF Receptor autophosphorylation in A431 cells.1-10 ng/mL of Recombinant Human EGF can effectively enhance EGF Receptor autophosphorylation.|Measured by its binding ability in a functional ELISA. Immobilized Human EGFR at 5 ug/mL (100 μL/well) can bind Human EGF with a linear range of 7-25ng/mL. Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.065-0.26ng/mL. |
Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
Reconstitution: | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
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