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Recombinant Human SCF/C-kit ligand Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 30-40 kDa.
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The purity of Human SCF/C-kit ligand Protein (Cat.RP00124) was greater than 95% as determined by SEC-HPLC.
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Recombinant Human SCF promotes the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.08-0.3ng/mL.
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Immobilized recombinant human SCF at 1 ug/mL (100 μL/well) can bind recombinant human CD117 with a linear range of 1-6 ng/mL.
Recombinant Human SCF/C-kit ligand Protein
Catalog No：AB-RP00124
Manufacturer：ABclonal
Synonyms：DCUA;DFNA69;FPH2;FPHH;Kitl;KL-1;MGF;SCF;SF;SHEP7
Expression Host：HEK293 cells
€135,00 – €355,00
| Product Name: | Recombinant Human SCF/C-kit ligand Protein |
| Category: | Proteins/Peptides > Other Cytokines & Cytokine Receptors,Cell Culture related |
| Description: | Recombinant Human SCF/C-kit ligand Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu26-His214) of human SCF/C-kit ligand (Accession #NP_000890.1) fused with a 6xHis tag at the C-terminus. |
| Gene ID: | 1324254 |
| Swiss prot: | P21583 |
| Bioactivity: | Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.08-0.3 ng/mL.|Measured by its binding ability in a functional ELISA. Immobilized recombinant human SCF at 1 ug/mL (100 μL/well) can bind recombinant human CD117 with a linear range of 1-6 ng/mL. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Reconstitution: | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
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