HCV Protease FRETSubstrate (RET S1
Catalog No：CP-HCVP-001B
Manufacturer：CPC Scientific
Size：1 mg
Formula：C68H89N 15O25S
MWt：1,549
€445,00
| Product Name: | HCV Protease FRET Substrate (RET S1 |
| Category: | Peptides |
| Three letter sequence: | Ac-Asp-Glu- Asp(EDANS) -Glu-Glu- Abu-蠄- (COO)Ala- Ser- Lys(DABCYL )-NH2 |
| One letter sequence: | Ac- DED(EDANS)- EE-Abu-蠄- (COO)AS- K(DABCYL)- NH2 |
| Description: | A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for |
| Literature References: | Lin, C. et al. J. Biol. Chem. 10, 1074 (2004); Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999); Liu, Y. et al. Anal. Biochem. 267, 331 (1999); Taliani, M. et al. Anal. Biochem. 240, 60 (1996). |
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