Western blot analysis of extracts of various cell lines, using DNMT3L antibody (A13591) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
DNMT3L Rabbit pAb
Catalog No:AB-A13591
Manufacturer:ABclonal
Synonyms:DNMT3L
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DNMT3L Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 151-386 of human DNMT3L (NP_787063.1). |
Gene ID: | 29947 |
Swiss prot: | Q9UJW3 |
Molecular weight: | 43kDa |
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