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Western blot analysis of extracts of various cell lines, using UGT1A1 antibody (A1359) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
UGT1A1 Rabbit pAb
Catalog No：AB-A1359
Manufacturer：ABclonal
Synonyms：UGT1A1;BILIQTL1;GNT1;HUG-BR1;UDPGT;UDPGT 1-1;UGT1;UGT1A;UDPGT1-1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | UGT1A1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5′ exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human UGT1A1 (NP_000454.1). |
| Gene ID: | 54658 |
| Swiss prot: | P22309 |
| Molecular weight: | 49kDa/59kDa |
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