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Western blot analysis of extracts of various cell lines, using SOD2 antibody (A1340) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Western blot analysis of extracts from normal (control) and SOD2 Rabbit pAb knockout (KO) 293T cells, using SOD2 Rabbit pAb antibody (A1340) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
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Immunohistochemistry of paraffin-embedded rat testis using SOD2 Rabbit pAb (A1340) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using SOD2 Rabbit pAb (A1340) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using SOD2 Rabbit pAb (A1340) at dilution of 1:100 (40x lens).
[KO Validated] SOD2 Rabbit pAb
Catalog No：AB-A1340
Manufacturer：ABclonal
Synonyms：IPO-B;IPOB;MNSOD;MVCD6;Mn-SOD;SOD2
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] SOD2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-222 of human SOD2 (NP_000627.2). |
| Gene ID: | 6648 |
| Swiss prot: | P04179 |
| Molecular weight: | 18kDa/19kDa/20kDa/24kDa |
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