Western blot analysis of extracts of various cell lines, using Ku80 antibody (A13369) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
Ku80 Rabbit pAb
Catalog No:AB-A13369
Manufacturer:ABclonal
Synonyms:XRCC5;KARP-1;KARP1;KU80;KUB2;Ku86;NFIV
Applications:WB,IHC
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Ku80 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 540-732 of human Ku80 (NP_066964.1). |
Gene ID: | 7520 |
Swiss prot: | P13010 |
Molecular weight: | 82kDa |
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