Western blot analysis of extracts of various cell lines, using MDM2 antibody (A13327) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
MDM2 Rabbit pAb
Catalog No:AB-A13327
Manufacturer:ABclonal
Synonyms:MDM2;ACTFS;HDMX;hdm2
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MDM2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-430 of human MDM2 (NP_002383.2). |
Gene ID: | 4193 |
Swiss prot: | Q00987 |
Molecular weight: | 11-14kDa/24-55kDa |
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