ABScript II Reverse Transcriptase
Catalog No:AB-RK21400
Manufacturer:ABclonal
Applications:DNA or RNA Ligases
€47,00 – €1.700,00
Description
Product Components
Components | Cat. No. |
---|---|
ABScript II Reverse Transcriptase (200, 000 U/ml) | AB-RM21400 |
5X First-Strand Buffer | AB-RM20109 |
100 mM DTT (10X) | AB-RM20117 |
Product Information
Featured | Specification |
---|---|
Product Source | The gene encoding a mutant M-MuLV Reverse Transcriptase (RNase H–) is expressed in E. coli and purified to near homogeneity. |
Applications | cDNA Synthesis, Reverse Transcription (cDNA Synthesis), RT-PCR & cDNA Synthesis , RT-qPCR, RT-PCR and cDNA Synthesis , PCR |
Unit Definition | One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly(rA)ooligo(dT)18 as template, with a total reaction volume of 50 μl. |
Storage Conditions | 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.01% IGEPAL CA-630, pH 7.5 @ 25°C |
Storage Temperature | -20°C |
Reaction Conditions | 1X First-Strand Buffer, 10 mM DTT, 200 units ABScript II Reverse Transcriptase, supplemented with 0.5 mM dNTPs (not included) and 5 uM dT23VN (not included). Incubate at 42°C for 50 minutes. If random primers are used, a 10 minute incubation at room temperature is recommended before transferring to 42°C. |
Reaction Buffer or Mix | 1X First-Strand Buffer Reaction Buffer: 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, pH 8.3 @ 25°C |
Heat Inactivation | 65°C for 20 min |
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