HpaI
Catalog No：AB-RK21155
Manufacturer：ABclonal
Applications：Restriction Endonucleases
€38,00 – €155,00
Description
5’…GTT/AAC…3′
3’…CAA/TTG…5′
3’…CAA/TTG…5′
Product Components
| Components | Cat. No. |
|---|---|
| HpaI (5, 000 U/ml) | RM21655 |
| 10X Buffer CutU | RM20102 |
Product Information
| Featured | Specification | ||||||
|---|---|---|---|---|---|---|---|
| Applications | Restriction Enzyme Digestion | ||||||
| Unit Definition | One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA fragments in 1 hour at 37°C in a total reaction volume of 50 µl. | ||||||
| Storage Conditions | 10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C | ||||||
| Storage Temperature | -20°C | ||||||
| Reaction Conditions | 1X Buffer CutU. Incubate at 37°C. | ||||||
| Reaction Buffer or Mix | 1X Buffer CutU: 20 mM Tris-acetate，10 mM Magnesium acetate，50 mM Potassium acetate，100 µg/ml BSA，pH 7.9 @25℃ |
||||||
| Heat Inactivation | 80°C for 20 min | ||||||
| Methylation Sensitivity |
|
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