NspI
Catalog No：AB-RK21124
Manufacturer：ABclonal
Applications：Restriction Endonucleases
€37,00 – €155,00
Description
3’…Y/GTACR…5′
Product Components
| Components | Cat. No. |
|---|---|
| NspI (10, 000 U/ml) | AB-RM21625 |
| 10X Buffer CutS | AB-RM20103 |
Product Information
| Featured | Specification | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Applications | Restriction Enzyme Digestion | ||||||||
| Unit Definition | One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. | ||||||||
| Storage Conditions | 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, 0.15% Triton X-100, pH 7.4 @ 25°C | ||||||||
| Storage Temperature | -20°C | ||||||||
| Reaction Conditions | 1X Buffer CutS. Incubate at 37°C. | ||||||||
| Reaction Buffer or Mix | 1X Buffer CutS: 50 mM KAc, 20 mM Tris-HAc, 10 mM MgAc2, 100 μg/ml BSA, pH 7.9 @ 25°C |
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| Heat Inactivation | 65°C for 20 min | ||||||||
| Methylation Sensitivity |
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| Activity in Abclonal Buffers |
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