Western blot analysis of extracts of various cell lines, using GLE1 antibody (A13206) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
GLE1 Rabbit pAb
Catalog No:AB-A13206
Manufacturer:ABclonal
Synonyms:GLE1;GLE1L;LCCS;LCCS1;hGLE1
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GLE1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GLE1 (NP_001003722.1). |
Gene ID: | 2733 |
Swiss prot: | Q53GS7 |
Molecular weight: | 75kDa/79kDa |
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