Western blot analysis of extracts of various cell lines, using U2AF1 antibody (A13166) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
U2AF1 Rabbit pAb
Catalog No:AB-A13166
Manufacturer:ABclonal
Synonyms:U2AF1;FP793;RN;RNU2AF1;U2AF35;U2AFBP
Applications:WB,IHC,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | U2AF1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U2AF1 (NP_006749.1). |
Gene ID: | 7307 |
Swiss prot: | Q01081 |
Molecular weight: | 8kDa/19kDa/27kDa |
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