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Western blot analysis of extracts of various cell lines, using EP300 Rabbit pAb (A13016) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human lung cancer using EP300 antibody (A13016) at dilution of 1:100 (40x lens).
EP300 Rabbit pAb
Catalog No：AB-A13016
Manufacturer：ABclonal
Synonyms：EP300;KAT3B;RSTS2;p300
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | EP300 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EP300 (NP_001420.2). |
| Gene ID: | 2033 |
| Swiss prot: | Q09472 |
| Molecular weight: | 264kDa |
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