Western blot analysis of extracts of various cell lines, using MRPS31 antibody (A12923) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
MRPS31 Rabbit pAb
Catalog No:AB-A12923
Manufacturer:ABclonal
Synonyms:MRPS31;IMOGN38;MRP-S31;S31mt
Applications:WB,IHC,IF
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MRPS31 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that has also been associated with type 1 diabetes; however, its relationship to the etiology of this disease remains to be clarified. Pseudogenes corresponding to this gene have been found on chromosomes 3 and 13. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 66-395 of human MRPS31 (NP_005821.2). |
Gene ID: | 10240 |
Swiss prot: | Q92665 |
Molecular weight: | 45kDa |
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