Western blot analysis of extracts of various cell lines, using POLE2 antibody (A12842) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
POLE2 Rabbit pAb
Catalog No:AB-A12842
Manufacturer:ABclonal
Synonyms:POLE2;DPE2
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | POLE2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human POLE2 (NP_002683.2). |
Gene ID: | 5427 |
Swiss prot: | P56282 |
Molecular weight: | 56kDa/59kDa |
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