Western blot analysis of extracts of various cell lines, using Brat1 antibody (A12801) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
BRAT1 Rabbit pAb
Catalog No:AB-A12801
Manufacturer:ABclonal
Synonyms:BRAT1;BAAT1;C7orf27;RMFSL
Applications:WB,IHC
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | BRAT1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this ubiquitously expressed gene interacts with the tumor suppressing BRCA1 (breast cancer 1) protein and and the ATM (ataxia telangiectasia mutated) protein. ATM is thought to be a master controller of cell cycle checkpoint signalling pathways that are required for cellular responses to DNA damage such as double-strand breaks that are induced by ionizing radiation and complexes with BRCA1 in the multi-protein complex BASC (BRAC1-associated genome surveillance complex). The protein encoded by this gene is thought to play a role in the DNA damage pathway regulated by BRCA1 and ATM. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 652-821 of human BRAT1 (NP_689956.2). |
Gene ID: | 221927 |
Swiss prot: | Q6PJG6 |
Molecular weight: | 30kDa/31kDa/88kDa |
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