Western blot analysis of extracts of various cell lines, using SP3 antibody (A12790) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
SP3 Rabbit pAb
Catalog No:AB-A12790
Manufacturer:ABclonal
Synonyms:SP3;SPR2
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SP3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-350 of human SP3 (NP_003102.1). |
Gene ID: | 6670 |
Swiss prot: | Q02447 |
Molecular weight: | 52kDa/53kDa/75kDa/76kDa/80kDa/81kDa |
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