Western blot analysis of extracts of various cell lines, using RAB7 antibody (A12784) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
RAB7 Rabbit pAb
Catalog No:AB-A12784
Manufacturer:ABclonal
Synonyms:RAB7A;PRO2706;RAB7
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RAB7 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human RAB7 (NP_004628.4). |
Gene ID: | 7879 |
Swiss prot: | P51149 |
Molecular weight: | 23kDa |
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