Western blot analysis of extracts of various cell lines, using GAR1 antibody (A12748) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
GAR1 Rabbit pAb
Catalog No:AB-A12748
Manufacturer:ABclonal
Synonyms:GAR1;NOLA1
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GAR1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-170 of human GAR1 (NP_061856.1). |
Gene ID: | 54433 |
Swiss prot: | Q9NY12 |
Molecular weight: | 20kDa/22kDa |
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