Western blot analysis of extracts of HCT116 cells, using CD133 antibody (A12711) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts from normal (control) and CD133 Rabbit pAb knockout (KO) HCT116 cells, using CD133 Rabbit pAb antibody (A12711) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] CD133 Rabbit pAb
Catalog No:AB-A12711
Manufacturer:ABclonal
Synonyms:PROM1;AC133;CD133;CORD12;MCDR2;MSTP061;PROML1;RP41;STGD4
Applications:WB,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] CD133 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 750-850 of human CD133 (NP_006008.1). |
Gene ID: | 8842 |
Swiss prot: | O43490 |
Molecular weight: | 92kDa/93kDa/94kDa/96kDa/97kDa |
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