.jpg)
Western blot analysis of extracts of various cell lines, using KRAS antibody (A12704) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts from normal (control) and KRAS knockout (KO) 293T cells, using KRAS antibody (A12704) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
[KO Validated] KRAS Rabbit pAb
Catalog No：AB-A12704
Manufacturer：ABclonal
Synonyms：KRAS;C-K-RAS;CFC2;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;KI-RAS;KRAS1;KRAS2;NS;NS3;RALD;RASK2;c-Ki-ras2
Applications：WB,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] KRAS Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-188 of human KRAS (NP_004976.2). |
| Gene ID: | 3845 |
| Swiss prot: | P01116 |
| Molecular weight: | 21kDa |
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