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Western blot analysis of extracts of various cell lines, using PSME3 antibody (A12697) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human breast cancer using PSME3 Rabbit pAb (A12697) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using PSME3 Rabbit pAb (A12697) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using PSME3 Rabbit pAb (A12697) at dilution of 1:100 (40x lens).
PSME3 Rabbit pAb
Catalog No：AB-A12697
Manufacturer：ABclonal
Synonyms：PSME3;HEL-S-283;Ki;PA28-gamma;PA28G;PA28gamma;REG-GAMMA
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PSME3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human PSME3 (NP_005780.2). |
| Gene ID: | 10197 |
| Swiss prot: | P61289 |
| Molecular weight: | 29kDa/30kDa |
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