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Western blot analysis of extracts of various cell lines, using PGK1 Rabbit mAb (A12686) at 1：1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
PGK1 Rabbit mAb
Catalog No：AB-A12686
Manufacturer：ABclonal
Synonyms：HEL-S-68p, MIG10, PGKA
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | PGK1 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014] |
| Immunogen: | A synthesized peptide derived from human PGK1 |
| Gene ID: | 5230 |
| Swiss prot: | P00558 |
| Molecular weight: | 45kDa |
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