Western blot analysis of extracts of various cell lines, using Occludin antibody (A12621) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
Occludin Rabbit pAb
Catalog No:AB-A12621
Manufacturer:ABclonal
Synonyms:OCLN;BLCPMG;PPP1R115;PTORCH1;occludin;Occludin
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Occludin Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-450 of human Occludin (NP_002529.1). |
Gene ID: | 100506658 |
Swiss prot: | Q16625 |
Molecular weight: | 8kDa/23kDa/31kDa/52kDa/54kDa/59kDa |
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