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Western blot analysis of extracts of various cell lines, using ASAH2 antibody (A12596) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
ASAH2 Rabbit pAb
Catalog No：AB-A12596
Manufacturer：ABclonal
Synonyms：ASAH2;BCDase;HNAC1;LCDase;N-CDase;NCDase
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ASAH2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008] |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human ASAH2 (NP_001137446.1). |
| Gene ID: | 56624 |
| Swiss prot: | Q9NR71 |
| Molecular weight: | 81kDa/85kDa |
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