Western blot analysis of extracts of various cell lines, using HP1 alpha/HP1 alpha/CBX5 antibody (A12592) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
HP1 alpha/CBX5 Rabbit pAb
Catalog No:AB-A12592
Manufacturer:ABclonal
Synonyms:CBX5;HEL25;HP1;HP1A
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | HP1 alpha/CBX5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human HP1 alpha/HP1 alpha/CBX5 (NP_001120794.1). |
Gene ID: | 23468 |
Swiss prot: | P45973 |
Molecular weight: | 22kDa |
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