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Western blot analysis of extracts of various cell lines, using HP1 alpha/HP1 alpha/CBX5 antibody (A12577) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts from normal (control) and HP1 alpha/HP1 alpha/CBX5 Rabbit pAb knockout (KO) 293T cells, using HP1 alpha/HP1 alpha/CBX5 Rabbit pAb antibody (A12577) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded mouse lung using HP1 alpha/HP1 alpha/CBX5 antibody (A12577) at dilution of 1:100 (40x lens).
[KO Validated] HP1 alpha/CBX5 Rabbit pAb
Catalog No：AB-A12577
Manufacturer：ABclonal
Synonyms：CBX5;HEL25;HP1;HP1A
Applications：WB,IHC,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] HP1 alpha/CBX5 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human HP1 alpha/HP1 alpha/CBX5 (NP_001120794.1). |
| Gene ID: | 23468 |
| Swiss prot: | P45973 |
| Molecular weight: | 22kDa |
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