Western blot analysis of extracts of various cell lines, using DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A12524) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
DNA topoisomerase I (TOP1) Rabbit pAb
Catalog No:AB-A12524
Manufacturer:ABclonal
Synonyms:TOP1;TOPI
Applications:WB,IHC,IP
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DNA topoisomerase I (TOP1) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (DNA topoisomerase I (TOP1)) (NP_003277.1). |
Gene ID: | 7150 |
Swiss prot: | P11387 |
Molecular weight: | 90kDa |
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