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Western blot analysis of extracts of various cell lines, using Akt2 antibody (A1250) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human breast using AKT2 antibody (A1250) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using AKT2 antibody (A1250) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using AKT2 antibody (A1250) at dilution of 1:200 (40x lens).
Akt2 Rabbit pAb
Catalog No：AB-A1250
Manufacturer：ABclonal
Synonyms：AKT2;HIHGHH;PKBB;PKBBETA;PRKBB;RAC-BETA
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Akt2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. |
| Immunogen: | Recombinant protein of human Akt2 |
| Gene ID: | 208 |
| Swiss prot: | P31751 |
| Molecular weight: | 51kDa/55kDa |
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