Western blot analysis of extracts of various cell lines, using PCNA Rabbit mAb (A12427) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PCNA Rabbit mAb
Catalog No:AB-A12427
Manufacturer:ABclonal
Synonyms:ATLD2
Applications:WB,IHC,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | PCNA Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human PCNA |
Gene ID: | 5111 |
Swiss prot: | P12004 |
Molecular weight: | 29kDa |
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