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Western blot analysis of extracts of various cell lines, using Clathrin heavy chain antibody (A12423) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Clathrin heavy chain Rabbit pAb
Catalog No：AB-A12423
Manufacturer：ABclonal
Synonyms：CLTC;CHC;CHC17;CLH-17;CLTCL2;Hc
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Clathrin heavy chain Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1451-1675 of human Clathrin heavy chain (NP_004850.1). |
| Gene ID: | 1213 |
| Swiss prot: | Q00610 |
| Molecular weight: | 187kDa/191kDa |
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