Western blot analysis of extracts of HT-29 cells, using APOBEC3A antibody (A12399) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
APOBEC3A Rabbit pAb
Catalog No:AB-A12399
Manufacturer:ABclonal
Synonyms:APOBEC3A;A3A;ARP3;PHRBN;bK150C2.1
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | APOBEC3A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human APOBEC3A (NP_663745.1). |
Gene ID: | 200315 |
Swiss prot: | P31941 |
Molecular weight: | 21kDa/23kDa |
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