Western blot analysis of extracts of various cell lines, using Alkaline Phosphatase (Alkaline Phosphatase (ALPL)) antibody (A12396) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Alkaline Phosphatase (ALPL) Rabbit pAb
Catalog No:AB-A12396
Manufacturer:ABclonal
Synonyms:ALPL;AP-TNAP;APTNAP;HOPS;TNAP
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Alkaline Phosphatase (ALPL) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human Alkaline Phosphatase (Alkaline Phosphatase (ALPL)) (NP_000469.3). |
Gene ID: | 249 |
Swiss prot: | P05186 |
Molecular weight: | 48kDa/51kDa/57kDa |
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