Western blot analysis of extracts of various cell lines, using UBA1 antibody (A12359) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
UBA1 Rabbit pAb
Catalog No:AB-A12359
Manufacturer:ABclonal
Synonyms:UBA1;A1S9;A1S9T;A1ST;AMCX1;CFAP124;GXP1;POC20;SMAX2;UBA1A;UBE1;UBE1X
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | UBA1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human UBA1 (NP_695012.1). |
Gene ID: | 7317 |
Swiss prot: | P22314 |
Molecular weight: | 113kDa/117kDa |
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