Western blot analysis of extracts of various cell lines, using POFUT2 antibody (A12223) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
POFUT2 Rabbit pAb
Catalog No:AB-A12223
Manufacturer:ABclonal
Synonyms:POFUT2;C21orf80;FUT13
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | POFUT2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-370 of human POFUT2 (NP_056042.1). |
Gene ID: | 23275 |
Swiss prot: | Q9Y2G5 |
Molecular weight: | 44kDa/48kDa/49kDa |
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