Western blot analysis of extracts of various cell lines, using SPOP antibody (A12106) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
SPOP Rabbit pAb
Catalog No:AB-A12106
Manufacturer:ABclonal
Synonyms:SPOP;BTBD32;TEF2
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SPOP Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 175-374 of human SPOP (NP_003554.1). |
Gene ID: | 8405 |
Swiss prot: | O43791 |
Molecular weight: | 42kDa |
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