Western blot analysis of extracts of HeLa cells, using STAT1 antibody (A12075) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
STAT1 Rabbit pAb
Catalog No:AB-A12075
Manufacturer:ABclonal
Synonyms:CANDF7;IMD31A;IMD31B;IMD31C;ISGF-3;STAT91;STAT1 alpha;STAT1
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | STAT1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 650-750 of human STAT1 (NP_009330.1). |
Gene ID: | 6772 |
Swiss prot: | P42224 |
Molecular weight: | 83kDa/87kDa |
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