Western blot analysis of extracts of various cell lines, using NELFB antibody (A12023) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
NELFB Rabbit pAb
Catalog No:AB-A12023
Manufacturer:ABclonal
Synonyms:NELFB;COBRA1;NELF-B
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NELFB Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 349-628 of human NELFB (NP_056271.3). |
Gene ID: | 25920 |
Swiss prot: | Q8WX92 |
Molecular weight: | 65kDa |
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